Ua hoʻopili immunosorbent mea o loko: palapala noi a me ka hiʻona o

Ua hoʻopili immunosorbent mea o loko - keʻena hoʻokolohua ao, i ua ma muli o ka 'ike o nā pono a pau o "antigen-lāʻau lapaʻau kino" me ka hana enzymatic lepili e hōʻike, ma ka hoololi ana i ke ano o ka kūpapakū. O ka papa kuhikuhi hana o keia Ka Ikepili - manao paa o ke alo o nā huahana o enzymatic reactions i loko o ka hoʻokolohua Eia kekahi laʻana.

No ka loaʻaʻana o nā antigens a me nā mea'aʻaʻa heterogeneous'ūhū immunoassay ua hoʻohana 'ia, mai kona kaʻina mea oluolu, e hookaawale i ka eiiiiiaiou o ka naoh, a i ka holo koke i kekahi o ia mau mea i loko o ka paa pae.

He waiwai 'ana i kumumanaʻo ka kēia pono o kēia keʻena hoʻokolohua mahele:

• kiʻekiʻe pā lonoaʻili, a hiki huai ae oia he uuku ka helu o nā lātoma o ka waiwai;

• no ka lawe ana mai i kēia Ka Ikepili hiki ke hoʻohana 'ia i ka palena iki helu o ka ho'āʻoʻia;

• i ka manawa kūpono o ka manawa lōʻihi, e mālama i nā eiiiiiaiou i mea pono no Ka Ikepili;

• Easy e hana;

• loaʻa nui o vocal a me ka pilikia makapō hopena;

• immunosorbent mea o loko ua hana nona iho ma kēlā me kēia kahua o ka naoh;

• kŘpa a haʻahaʻa kāki He poho i i hoʻohana no kaʻike,.

Kēia mau pono e nui ana o IFA i loko o kekahi mau wahi o ka oihana lapaau, a me, no kaʻike, o nā mea hoʻomaʻi.

Ka loa, he pono ole i ke kaʻina hana no ka immobilizing nā mea'aʻaʻa a me antigens mea he adsorption naoh, i ka wa e pili ai ka kaʻawale nā lātoma a hiki i ka paa pae (ma muli o ke ionic a hydrophobic hana kūleʻa paha ma ka ho okumu ana o hydrogen paa).

Au pono aku nei au i ua hoʻohana 'ia ka'ūhū immunoassay i loko o nā hihia:

• e kuhikuhi i antigens o pathogens o likeʻole nā mea palahēhē;

• e hooholo i ke alo o nā mea'aʻaʻa i ka antigen o pathological microorganisms (ka mea e e hoʻomaopopo i ka Elisa ke huai ae aneane kekahi wahine maʻiʻaʻai).

Special polystyrene papa i hoʻohana 'e lawe mai i kēia serodiagnosis. Ua loaʻa 96 puka ma ka aoao o adsorbed antigen. I komo i loko o ka papa hoao aku la ia aeea antigen nā mea'aʻaʻa i i pili kūpono. Ka hope hou ana o ka 'ae' lāʻau lapaʻau kino a hiki i ka pūnāwai o kanaka immunoglobulins kŰlń ao kekahi naoh. Ma ke kēia iapaau ana lāʻau lapaʻau chromogen (kalameaʻai) loli aʻe 'ia nā pōpō kūpapakū (i loko o ke alo o ka like eiiieaenu "antigen-lāʻau lapaʻau kino"). Ua mea waiwai 'ana i kumumanaʻo i ka waihoʻoluʻu pinepine' mea pololei hapaʻauhau i ka nui o nā mea'aʻaʻa.

Elisa Kńlailai nā kūlanaʻelua ana i ka'ōnohi nuʻa o ka wai i loko o ka punawai. kūikawā manaʻo a stereotyped lauana e kōkua helu i ka lāʻau lapaʻau kino lehulehu Penei ke hoʻohana 'ia. Ua hoʻomanaʻoʻia ai ia i kela hoʻokolohua nenoaiu e hoʻomaka i kēia Ka Ikepili i kona aiee o maʻamau nā hoailona (keia ua ana i koi 'ia i loko o nā hualoaʻa, i ae ai ua haawiia mai).

E ho ohana i Elisa diagnose syphilis, mea hoʻomaʻi hepatitis a me HIV, e like me loaʻa i loko o ka kanaka kino cytomegalovirus, Chlamydia, herpes (ʻano). Kēia Ka Ikepili kekahi kōkua e hooholo i ka nui o ka hormones, e huai ae maʻi 'aʻai hōʻailona.

Where no ka hoao ana ma Moscow? I kēia lā, aia nō he wahi lihi iki o ka hana kūikawā keʻena hoʻokolohua, a kaumaha aku i ka laulā huahelu o serological ho'āʻo ma. No kaʻaoʻao e nīnau aku i ke kauka o ka kūloko? Aaieou (a pilikino paha i 'oihana, e dveryaete). Me ka hoao ana i nā hualoaʻa no hoi e hele aku i ke kauka. A hoʻolohe i kona manaʻo ma luna o ka pono no kekahi lapaau.