Ploskireva meakino (Bacto-Hagara F): hōʻikeʻano. Lapaʻau o microbiology

Ploskireva meakino (i hoakaka ia i oleloia e like malie Bacto ua Hagara la F) mea he nutrient meakino no ka mahi ana i kekahi mau microorganisms, kuhikuhi Shigella a me Salmonella. E like me ke kumu no kona hana iho mailoko o kekahi mau kumuhana: mimi, bile, feces.

moʻolelo

Nikolay Ivanovich Ploskirev - Soviet microbiologist a me ka hoʻonaniʻiaʻo ia kauka. He Ua hanau ia ma 1869, a make i ka 1948, ua hana loa o kona ola i loko o kona aina - Tomsk.

I ka 1888,ʻo ia, i ka wā e hiki mai nä känaka 'epekema, me ka pau i ka ono o ka papa o ke kula, hui i ka poe koa. ka mea, ua hiki ke hoʻokō 'ia i ke aʻo wale i ka 1892, a laila, hele akula nā examinations no ke komo ana i ka imperial Tomsk University,ʻo ia aʻo ma ka'āuna kumu o Medicine. I ka 1898, ua loaa mai i ka inoa o ke kauka.

Ploskirev komo i loko o Lūkini-Japanese War, a ma hope o ka mea hana ma ka Tomsk haukapila. I ka 1910, ua hoonohoia ma ka lāʻau kū o ka poo o ka Tomsk dermatological a me ka venereal haukapila, a no kanakolu makahiki i hala aku kona mau poo. Ua like nä känaka 'epekema i kekahi o na Mamua o STI? Aaieou ma Tomsk.

Nikolay Ivanovich Ploskirev, palapala iho la i ka moʻo o ka hana hoolilo a hiki i ke kaua ana i ka venereal maʻi.

'ole

Wednesday Ploskireva - mea no ka mahi ana i intestinal koʻohune, a, no laila, pono no kekahi mau ano o ka mahuaola. Ua ua hua mai la i ka maloo palapala.

Muli o kona kulana he nui ke ano o ka huina kaumaha mea pancreatic hydrolyzate sprat (10.4 g. / H). Iki emi manaoia no hydrocitrate dibasic paʻakai (8.5 g. / H). No hoi he mea he maloo nutrient kai a me ka waiū kō (8.62 a 7,3 g. / H).

Ka lua o ka inoa Ploskireva meakino - J. Bacto-Hagara i loko o ka haku mele 'ana ia mea i loaʻa i loko o ka nui o ua Hagara la, 6,94 g. / Ho'āo ai. Seriovatistokislogo anhydrous paʻakai maʻiʻo like i 5.1 g. / Ho'āo ai. Aia o ke alo o anhydrous disodium posapahate - 2.1 g. / L, paʻakai paʻakai o cholicʻakika ma kahi o 3,46 g. / K a me ka soda lehu - 2.4 g.

Emi iho malalo o ka gram, he ma laila nā hoailona -'ākilikaiʻulaʻula (0.05) a me ka maikaʻi loa uliuli (0.0002). Ka iodine maʻiʻo - 0,13.

palapala noi

Radio ka like ana o ka biochemical keʻano o ka hanaʻana o nā koʻohune he kūlana loa nui i loko o kaʻike, o ka maʻi i i anaerobic meaola. Laha 'i loko o kēia' ohana - haneri. Ka mea, e kokoke'ālike ma ka morphology a me ka nohona waiwai, a no ka maopopo ia lakou, mai kekahi i kekahi loa ike loea ala - o ke ao o ka biochemistry.

Ka like hoomaka me ka inoculation o pathological mea i loko o ka nohona meakino. Ua pathological palena pau no ka coliform koʻohune a me kekahi hapa o ka mea, ma ka hou i ka papu ua Hagara la, ka loaʻa o ka malamalama a me ka lactose. Aaioee hiki o lactose - nui ka hoailona o ka differentiation o enterobacteria. Inā keia ua loaa, i ka pH hoʻololi ai i kaʻakika a me ke anakahi ua ho'ā 'ia paha, i huli mai i ka panalaau ia.

Other'āina i hoʻohana 'ē aʻe nutrient Media, akā, i ko lākouʻano papa hana o ka' ana hoʻopili like i ka ekolu loa, he pono ole i loko o Rusia - keia 'ia Endo a me Levine Ploskireva Poakolu.

Description o ka iaoiaeii

Abroad Ploskireva Analog meakino mea he ai-kapaia Macconkey Hagara. I ka pau palapala aniani moakaka pāʻoihana i ke kukui a pink-yellow waihoʻoluʻu. Panalāʻau hiki i ke kō lactose, i waena o Ploskireva haawiʻulaʻula (cranberry) waihoʻoluʻu. Inā ka koʻohune hiki ole ke kō ia, a me ka panalaau ia mea colorless paha i ka nawaliwali waihoʻoluʻu.

Ua haawiia i ka haku mele 'ana o ka meakino nā waiwai Ploskireva nā mea kūpale (''he'ōmaʻomaʻo, bile paʻakai, iodine), ka mea, ua aneane loa inhibits ka ulu o ka gram-oluʻolu flora, e like me ia ma ka mua lā eshehiry me ka nui loa retards ka ulu, e like me nā hana mau concomitant microflora.

lua o ka wāwae

Hou ia o ka hoihoi o ka koho o ka panalaau ia, a me ka mea, ua mau keiki ma luna o ka iniiaiie pūnaewele meakino a me ka differentiation. 'Ia i loko o i loaʻa ua seeding e komo mau substrates. Mea koʻohune moʻomeheu e huai ae enzymatic hanana hulina me ka pili ana ia ia, a hiki i ka ia meakino waiho i loko o ka paipu no laila, ua loaʻa nō nā kahua:

• kekahi ma i ka naʻe o ua Hagara la;
• me ke kia ao.

Ke kuleana o ka noiʻi i loko o ka panalaau ia na kanaka] bevelled hapa anuanu hahauʻana, a ma ke kia ao - he mea oioi. Ma keia ke kahua, ka meakino hoʻohana Russell, Kligler a Olkenitskogo. Like palena pauʻike, hoʻohana a me Ploskireva Poakolu.

Microbiology a pathogenic meaola

Hagara Ploskireva mea nui he ololi i loko o kaʻimiʻana nā mea palahēhē, kena ae la ia e Enterobacteriaceae. No laila, no ka laʻana, ka mea, ua ulu a ua differentiated panalāʻau o microorganisms a i bacillary ka hī koko. Kēia anaerobic meaola pili ana i ka genus Schigella.

Like a pau e ka hapa o ka ohana o intestinal koʻohune, shigella i ke ano o ua mau laau la, i ka nui o nā micrometers. Ka mea, e i hoolilo capsules a me spores i flagella - ia e leie aku ia ia e ike okoa mai Salmonella, a, ma ka huli, e lawe lima. Ka mea, ulu pono loa na mea Media ma luna, ma ke ana wela iki ma luna Ambient (35-37 ° C) a 7,4 pH. Ma ke ano o ka ulu, panalāʻau mai i oko mai Salmonella.

E like me kūkā luna, microorganisms hiki e kūpono kou morphological oko ao, nae, oko a me ka nui loa i loko o biochemical keʻano o ka hanaʻana o ke ola. Penei, koe wale no ka Shigella Nyukestl, me ke kahua o kaʻakika kinoea ia lŘlŘ i fermentation o carbohydrates. Me ke koe o Shigella sonnei, ka mea hiki ole kaʻawaʻawaʻana lactose, akā, ua hōʻuluʻulu manaʻo monakō koko. Pono i kā lākou mau kī hiʻona, e ke lawe ia mea ka mea, e hiki e ho'ēmi i niraty i ka nitrite. Ua hōʻuluʻulu manaʻo lākou mau panalāʻau me ka urea, e like me ka pono me ia ma ka moʻomeheu meakino mea ole liquefaction o gelatin.

Collection a me ka makaukau o ka hua

E huai ae konohiki o ka hī koko, i koi microbiological ho'āʻo ai i noho ai hana kūpono, oa faeces ahonui. Ma ka loaʻa 'ana o ka mea, e pono like' āwīwī i hiki e hana i ka hua. Inā keia mea,ʻaʻole e hiki, pono e waiho i ke kumu i loko o ka preservative - posapahate aooa? Läÿau, a me glycerol. At 4 ° C, ka mea hiki e waiho waho no ka oi ma mua o ka lā. Ohi i ka mea pono e lawe mai ma ka mea o ka rectal aniani şarkı i ua hookomoia iloko o ka rectum.

No ka haʻawina o ka mea i koho purulent mucus'āpana e e holoi i mau a hiki i ekolu paipu isotonic paʻakai koloriside pāʻoihana.

Noi o pathogenic koʻohune i loko o ka papa Ploskireva i aniani spatula. Oe Pono e hamo i ma luna o kekahi hapa uuku o kona Hagara, alaila, haehae iho la i ka spatula ma ke kaiapuni, a ua hamo i ke koena mea i loko o kaʻili lūlū. Inā ka seeding ua hana ia ma kekahi mau kīʻaha, a laila, i kēlā me kēia no ia e pono ai, e kanu iho i ka hou'ōhui.

Inā muco-purulent apana ma laila i loko o ka lawe ana i ka mea laʻa Ka Ikepili, ka mea, 'aʻole i manaʻo i ka mea, aole imua o ke pathogens. Ma ia he hihia ia mea pono e emulsify feces i loko o 10 ml o ka paʻakai koloriside (lehulehu 0,85%), a laila, kanu i kekahi paha mau kulu ma kekahi meakino Ploskireva. Ole-emulsified noho hiki e lūlūʻia i loko o selenitovy kai. He i hoʻohana ', ina, kahi o na feces i luaʻi paha holoi ana.

Microbiologicalʻike,

Ma ka mua kahua o microbiological haʻawina lawe mai lulu o pathogenic koʻohune i loko o nā kīʻaha, a laila, e kiaʻi ana i ka ulu Shigella. Ma Wednesday Ploskireva hua mai la i kaʻai i waena, a me ka lua o ka - ma ka hoʻolilo 'ia a me Endo Levin.

Aie i ka mea e noho kokoke ana ke kanana nei o ka shigella e hoʻopale ai i antibiotics, i Ua inā i ka meakino me ka chloramphenicol. A laila, ia ia lŘlŘ loko lā incubation i loko o ka incubator ma 37 ° C.

Ma ka lua o ka lā, e makaikai i ka ulu panalāʻau. O ka mea e ole ana ma ka palena pau PalekanaEND_LINK meakino ulu colorless, ia mea pono i ka nāhelehele i mai Russell ka meakino a pōkole "motley lālani." E möakäka hou noiʻi ua i lawelawe 'ia e ka algorithm o ka iniiaiie ulu ma luna o lākou. Panalāʻau o Shigella ma ka Poakolu Ploskireva ulu i loko o ka palapala o ka aniani moakaka, colorless a me ka uuku kolamu. Ka mea, eia no ÿelua:

• flattened me kukū lua penei;
• ua hoʻokōʻia, convex, me ka typical pulu alohi.

Ekolu a eha panalāʻau pono e mikroskopirovat a hookolokolo aku la ia i ka hoʻoneʻe. Inā i ka la mahope, aole ia e hōʻike mai, relocated ka mea, ma ka Wednesday Olkenitskogo e kaʻawale i ka nohona maemae. I ka wa e kaawale aku o ano panalāʻau Shigella a ina aole ka ulu ua malama i na mea a pau, ka mea, he pono ke Endo Hagara a Ploskireva hua seeding o selenitovogo kai. Inā ka typical panalāʻau e noho ana i loko o lawa helu, e kau i ka oaeuiinoe agglutination. A hui o sera sonnei a me Flexner, naoh ia lŘlŘ ma ke aniani.

hou noiʻi

Ma ke kolu o ka lā, aia no na loli ana i hanaia i loko o ka panalāʻau o ka meakino Russell ka. Inā he mea i ka moʻomeheu i pili ole degrade i ka lactose a me ka monakō koko ferments e paka i kaʻakika, ka mea, ua hoʻokaʻawaleʻia, a ua kālailai, pana aku au i microscopy, e like me ka lawe ana mai lulu ana ma ka "motley lalani," akā, i kēia manawa hoʻonui. Iki i ka agglutination naoh, ma ka mea i serological 'ike.

Ma ka lā hope, e hiki ke manao ma ke kumu o ka loli ma ka "motley helu" (ina he mea fermentation o carbohydrates), e like me hōʻuluʻulu manaʻo no ka ka agglutination naoh.

Pūnaewele a me ka makaukau

Makaukau o Hagara Ploskireva ia lŘlŘ like penei:

1. 55 g. Maloo waiwai ua ho'āla i loko o ka hano o ka i puhi ia wai.
2. Hoolapalapaia i mau a hiki i 'ekolu minuke, a hiki i ka Hagara la oia ke ole nae loa hehee.
3. E ninini i loko o petri mau (sterility koho) ahu iho la ka 5-6 mm o.
4. e waiho hamama nā papa no ka hapalua hora i mehana ea (18-25 degere). Ma hope iho o kēia manawa i ka meakino mea nui kūpono Mai hoopaakiki a me ka maloo.

Wednesday Ploskireva photosensitive a me ka hygroscopic. Ka pauka E e waiho ia ma ka hoailonaia he AEeAaOA, ka hoahanau humidity i loko o ka lumi pono i oi aku mamua o 60%. ʻO kaʻoi loa ana wela no ka papaa o ka 2 ° C i 25 ° C. Waho pūnaewele Pono e hoʻokō i kēia mau rula, i ole ia e hiki paʻewa ai nā haʻina.