Vitrification o embryos ma ke kanaka nā moku halihali mokulele

Ma kēia 'atikala e kamaʻilio pū mākou e pili ana i na mea o ka manaʻo like vitrification o embryos. Dr Kuwayama Masashige i invented i kēia papa hana, e kriotopah a hiki i ka makahiki makahiki. Ka mua ke keiki i hanau ma vitrification embryos i ka 2003. Ua hoomahuahua ia ka 98 pakeneka oocyte ola kōmi.

Hapalua o ka wahine ka poe i lawe IVF, na embryos koe. No ia paa ana cryopreservation e leie aku nā mea maʻi e hoola i ke kālā. Ma hope o nā mea a pau unfreeze, a hoolilo i ka embryos ka nui maʻalahi ma mua, e lawe mai i loko o lūmaua fertilization ina hana hou. Ua mea no hoi kekahi ano o ka lapaʻau ma ka hihia ina he wahine mea i hapai. Cryopreservation i He okoa lanakila - hikiʻole i ka make ana o viable embryos i koe ma hope o ka Hola Pūnaewele.

ontogeny

No ka mea pilikino kūpono 'ana o ka meaola, a ontogeny, e nā lā hoʻi, mai ka manawa mai o fertilization a me ka welau a me kona make ana. Kēia noi i ana i ka manawa a me ka mai he mau külana. A ua hiki ole i kona, e ke wehe 'ia ai' ūlōlohi mai ana kona ulu ana. Akā maoli ai, ua hewa. ¶ʻO ia nā mea kanu, invertebrates, a hiki i kekahi kula haʻahaʻa vertebrates mea i haʻahaʻa wela, mai ho'āʻo waiwai ano o ke ola meaola.

He aha ka hibernation?

Vitrification o embryos kūkā mai ma lalo nei. Kanaka wā o ka malie ua kapaia hibernation. No kekahi laʻana, na holoholona ola mahope aku o ka Siberian mahana hiki -90 degere, a me ka aneane piha dehydration. Ma ka hoʻopaʻa 'ana o kēia wā o ka ontogenesis ma ka' ano he mea he ninau e pili ana i ka hiki ana o ka haʻahaʻa mahana no ka hapa 'a me ka reversible haʻahaʻa o ka functioning o ke kiekie vertebrate mea, me kānaka.

cryopreservation

Cryopreservation - mea he pono ka hana no ka wehe ana o liluwelo keʻano o ka hanaʻana i loko o keena ma ka waiū i haʻahaʻa ana wela. Kēia e mālama ana i ke ola ha awina o ke keena i paio hahana maoli nō. By hiʻona, keia iaoia mea emi iki mahope o embryo vitrification. 1 kriotop ( 'ae' krionositel) kekahi, mai 1 a 3 embryos.

No ka laʻana, ka wā hoʻokō i ka hana e like me IVF, me ka maikaʻi hookoia ua manaoia e e neʻe maila i loko o ka uterine lua mea i oi aku mamua o elua embryos. Ka i koe e like ai embryos hiki ke cryopreserved no ka wā e hiki mai ana. Ka mea, ke i pili no ka hou-IVF ma hope o ke ana, ina o ka hana, e hoike aku i ka io result. Me ia mau pahu hopu lawe vitrification embryos ma luna o kanaka nā moku halihali mokulele.

Ma kekahi mau hihia, pa ahau a pau embryos. No ka wahine, ka poe i ovarian hyperstimulation maʻi pale i superovulation hoʻokomo ua hana loa pinepine ana. ʻO wai ka mea i paipai ke kūpaleʻana? Nā mea maʻi pilikia mai ka maʻi 'aʻai, ma ka pau mua o ka kaʻina hana chemotherapy paha radiotherapy. Keʻikepili Ua laila hoololiia embryos i loko o ka uterine lua. Pa ahau ke hōʻike a pau i ka manawa o ka hāpai keiki ma hope o IVF no kekahi mau kumu kuu iho la. Kēia paha e endometrial polyps, lawa endometrial mānoanoa i ka manawa hoʻopaʻa 'hoolilo, dysfunctional kahe ana ke koko.

ke kūpaleʻana kahua o ka hoʻonaʻauao

Nā embryos Ua paʻahau ma kekahi mau kahua o ka hoʻonaʻauao.

• fertilized hua (zygote);
• palaoa ke kahua embryos;
• blastocysts.

I ka manawa, he mea mau ano o ke kūpaleʻana embryos.

kali ke kūpaleʻana

Vitrification o embryos ua lawe mai i ke kali ke kūpaleʻana. Oia kekahi papa hana i manaoia ma na 70s a me kekahi o ka mua panina ano o ke kūpaleʻana embryos. Ua Ua nānā 'ana i lohi ka huʻihuʻiʻana i kekahi mau kōmi. Once e waiho na embryos i loko o ke kinowai ai naikokene.

Akā, he mea pono e kaulana ia ma na mea ke kūpaleʻana i loko o ka cryoprotective pāʻoihana microscopic hau nā pōaka i hana a he kūlana maikaʻiʻole i nā keena o ka embryo. Ua hiki i ka helehelena a huina pau loa ia'na o liluwelo nā luna o paio hahana maoli nō. Ka helu o nā embryos ana hoololiia i ka lohi ke kūpaleʻana a me ka thawing, ka hola 70 pakeneka.

vitrification

Ma hope o ka makahiki 2010, hoomaka ae la e pili hou oi pono, a me ka ano o ka cryopreservation - vitrification. Ke hoʻohālikelike a me ka mea mua iaoia, keia mea he Naha Pōhaku-hookeai ala e pa ahau ke liluwelo mea. Loa pinepine lawe embryos ma hope o vitrification PGD (aaiaoeʻike,).

I ka hoʻohana 'ana i kēia kaʻina hana cryoprotective pāʻoihana, kahi embryos e hoonoho iho, aole ia i hana i hau nā pōaka i ke kūpaleʻana. Penei, hoemiia outage pahiki embryo. Ke makakoho o keia iaoia mea ole wale i kaʻaoʻao o ke kūpaleʻana, akā, i ke ola ana o embryos ma hope o thawing. E like me ka 'ikepili helu, ka helu o ke koena embryos ma hope o vitrification kaʻina mea ole e emi malalo o 95 pakeneka.

E ahaʻia ana ma hope o thawing?

Ma hope o thawing na embryos mai i oko mai maʻamau embryos. Ka mea, e like me ka pono lawe kumu a me ka ulu. Ma ka hahana maoli nō kēia a pau embryos malalo iho a hiki i ka kokua hatching. Ma alakaʻi kēia hana o kaʻilikai o ka embryo ua maheleia kukuna mea nana i ulana i ka makemake huina a me ka maluhia. Kēia kōkua ai i nā ka embryo mai o ka iwi, a maoli he i ka manawa kūpono o ka holomua transplantation i loko o ka uterus.

Ke kūpaleʻana embryos apono 'waho pūnaewele no ka lōʻihi manawa. Kēia kaʻina He kāki-mana, no ka mea, i ke kumu kūʻai o ke ola, a me ka thawing e kanu pūʻia me ka embryo i loko o ka uterus mea i emi ma mua o ka lua o ka kaʻina hana o ka loko o lūmaua fertilization.

Vitrification ua noʻonoʻo me kaʻelua hoʻololi kahi i ke anu pāʻoihana ma lalo anuanu maila ma lalo o ka aniani hoʻololi mahana. I ka Ia manawa e koe amorphous 'ole o ke aniani, a loaʻa i ka mea e like ai i like i ka mea pōaka puaʻa hiwa. Penei, o na keena e noho ana, a hiki i ka pau embryo ia iho i loko o "aniani". ua loaa Glassy 'ole o ka wai ma lalo o vitrification no i kona kokololio ka huʻihuʻiʻana, ua hoemiia a laila entropy o ka wai i loko o ka wā pōkole o ka manawa ma mua e pono ai entropy aniani' ole.

Ma na mea olelo, i ka wai a 'aʻole i pa ahau ina kona entropy hoʻokokoke e entropy aniani. Akā, ma ka mea e pono vitrified ola meaola, ia mea e pono ke kau i ka mämä holo o ka emi o ke ana wela ≈ 108 ° C / min., A he mea hiki ole i ka hana, no ka mea, ka mea noi cryogenic wai a mea,ʻaʻole lawa no keia wela, aʻaʻole hiki ke hoʻohana 'vitrifitsiruemy pāʻoihana i loko o ka uuku buke ma mua o ka buke oocyte. Keia mea a pau paipai a me ka vitrification o embryos. He aha ka mea, e ka manawa oi a emi kālaʻe.

ua hiki ke hooiaio i na mea ulu ma ka Wednesday, e pa ahau ke cryoprotectants e leie aku oe e koke lalo loa i ka ke kūpaleʻana māmāʻepekema. Nolaila, ma ka pa apŘ o 10% propylene glycol a me ka etilengligolya holo ua nui hoemi, ma ka 40 pakeneka o ka pa apŘ o ke vitrification mea hiki i ke anuanu maila uku o 10 ° C / min, a ma ka 60% uku Hawaiʻi i 50 ° C / min. Akā, me ka mea i loaʻa i loko o nuʻa o cryoprotectants, haule ma Wednesday, mahuahua ko lakou hopena maikaʻi 'ole ma o ka ke kūpaleʻana o biomaterials. Kali ke kūpaleʻana hoʻonāukiukiʻana mai o hoʻomāhuahua o kapa wai i loko o ka liluwelo meaola a me intracellular hehee ai. Oia ke ano ia lŘlŘ pono i ka loa dehydration keena me ke ano o ka extracellular hau.

Alaila, ma ka loaa ana o ka vitreous hale, mai hemo mai Nine Inch a me physical keʻano o ka hanaʻana o ka dehydration. I loko nō o ka mea i ka vitrification o embryos (ia mea, ka mea, ua ho'ākāka 'ia ma ka au mamuli luna) mea nani paakiki physical'ōnaehana, hiki ke loaʻa pono o kēia' ole i loko o kā mākou kela la i keia ola (aniani, silikone, a no laila, ma luna).

Vitrification o embryos: hōʻike manaʻo

Kēia hana 'ohi i maikaʻi wale nō manaʻo. vitrification ke kaʻina hana no ka hiki. Akā, ka mea, i ka hailonaʻana o nā hiʻona i loko o kahua o ka hoʻonaʻauao likeʻole o ka? Acaeoey i loko o IVF keʻena hoʻokolohua. Vitrification - mea ole hou kekahi ano o ka cryopreservation o ola keena. He mea ka hope koe o kali ke kūpaleʻana. I kēia lā, e hiki no i ka bebe, no ka 'epekema Aey? Acaeoey na wahine.

mea, ua ulu

Aie i ka hana ana i na mea he nuiʻepekema vitrification hiki ke hana me ka hoʻohana 'ana o ka pipiʻi hanaʻia pahu hau a me ka hoʻohana' ana i na mea lako a pan e nā kahu nānā i ka Aʻole. Penei, ka Nohie iaoiaeii a me ka hou hoʻokūkū. I loko nō o ka hana 'ia he nui i loko o cryopreservation, i ka manaʻo o ka pono mālama o ola meaola i haahaa ka mahana i keia mea hiki ole.